FOXD3 promotes homologous recombination repair and genomic stability by facilitating MRE11-mediated DNA end resection
Homologous recombination (HR) is crucial for the high-fidelity repair of DNA double-strand breaks (DSBs), ensuring the maintenance of genome stability. In this study, we show that FOXD3 interacts with poly (ADP-ribose) polymerase 1 (PARP1) and is recruited to DSBs in a PARP1-dependent manner. FOXD3 directly binds to the DSB repair protein MRE11 and promotes its recruitment to DSB sites, ensuring proper end resection. Inhibition of FOXD3 expression compromises HR-mediated DSB repair and chromosome stability and sensitizes cancer cells to ionizing radiation. Collectively, our findings demonstrate that FOXD3 promotes HR-mediated DSB repair and genome stability.
Authors
- Ziyi Xiong (ORCID: https://orcid.org/0000-0002-2361-2849)
- Congwen Gao (ORCID: https://orcid.org/0009-0006-6622-9109)
- Shibin Xu (ORCID: https://orcid.org/0000-0003-4146-2466)
- Xingzhi Xu (ORCID: https://orcid.org/0000-0002-4459-2082)
- Yamin Gong
- Jingyu Zhang (ORCID: https://orcid.org/0000-0002-5057-5553)
- Hongxiang Chen (ORCID: https://orcid.org/0000-0003-0989-3565)
- Bao Chai
Institutions
- Union Hospital (HK)
- Shenzhen University (CN)
- Wuhan University (CN)
- Zhongnan Hospital of Wuhan University (CN)
- Huazhong University of Science and Technology (CN)
Publication Details
- Journal
- Acta Biochimica et Biophysica Sinica
- Published
- 2025-04-16
- DOI
- https://doi.org/10.3724/abbs.2025063
- Primary Topic
- DNA Repair Mechanisms
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Natural Science Foundation of China
- Huazhong University of Science and Technology
- Shenzhen University
- Shenzhen Fundamental Research Program