Facile DNA chemical ligation under mild conditions enabled by 1-cyano-4-(dimethylamino)pyridinium tetrafluoroborate (CDAP)
We report the chemical ligation of DNA using 1-cyano-4-(dimethylamino)pyridinium tetrafluoroborate (CDAP). CDAP promoted DNA ligation without depurination or undesired side reactions to the nucleobase. Ligation of 3'-phosphate and 5'-hydroxyl termini was achieved at low temperatures with good yield. This study provides a novel method for the synthesis of artificial oligonucleotides.
Authors
- Hiroshi Abe (ORCID: https://orcid.org/0000-0003-0048-3789)
- Yasuaki Kimura (ORCID: https://orcid.org/0000-0002-3609-602X)
- Hiroki Yamada (ORCID: https://orcid.org/0009-0009-0231-2706)
- Junichiro Yamamoto (ORCID: https://orcid.org/0009-0008-9188-0715)
Institutions
- Kyowa Kirin (Japan) (JP)
- Japan Science and Technology Agency (JP)
- Nagoya University (JP)
Publication Details
- Journal
- Organic & Biomolecular Chemistry
- Published
- 2025-01-01
- DOI
- https://doi.org/10.1039/d5ob00411j
- Primary Topic
- Advanced biosensing and bioanalysis techniques
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Japan Agency for Medical Research and Development
- Japan Society for the Promotion of Science