Label-free method to monitor metabolism during long-term culture of human pluripotent stem cell derived cardiomyocytes
Our results demonstrate that label-free OMI can be used to monitor metabolic changes in hPSC-CMs under varying culture conditions in a rapid, non-destructive manner with single-cell resolution, providing insight into metabolic transitions arising from time in culture, culture conditions, or disease states.
Authors
- Danielle E. Desa (ORCID: https://orcid.org/0000-0001-8975-0526)
- Xiaotian Zhang (ORCID: https://orcid.org/0000-0002-9533-4761)
- Sean P. Palecek (ORCID: https://orcid.org/0000-0003-4917-5584)
- Tongcheng Qian (ORCID: https://orcid.org/0000-0002-9071-3543)
- Melissa C. Skala (ORCID: https://orcid.org/0000-0002-6320-7637)
- Emmanuel Contreras Guzman (ORCID: https://orcid.org/0000-0002-1929-3961)
- Wenxuan Zhao (ORCID: https://orcid.org/0009-0004-1952-6093)
Institutions
- University of Wisconsin–Madison (US)
- Morgridge Institute for Research (US)
Publication Details
- Journal
- Biophotonics discovery.
- Published
- 2025-04-17
- DOI
- https://doi.org/10.1117/1.bios.2.2.025001
- Primary Topic
- Pluripotent Stem Cells Research
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Institutes of Health