Genetic Variability for Yield and Yield Contributing Traits of Chickpea (Cicer arietinum L.) through SSR Markers

The molecular genetic diversity analysis with six chickpea mutants of genotype-V2 (IC265298) with one control (untreated) to determine their transferability in chickpea, 24 SSR markers taken from adzukibean, common bean, cowpea, mungbean, chickpea, and pigeonpea were employed". The effectiveness of transferable SSRs was also examined in several genotypes of this species". The results revealed among the twenty-four SSR markers utilized, a total of 71 alleles were amplified, with the number of alleles ranging from 2 to 4 and a mean of 2.63 alleles per locus. Particularly, highest (4) number of alleles were amplified by markers SSR 1,4. The amplified products with 24 SSRs were ranged from 116 to 277 bp. The highest PCR fragment (277 bp) was generated by primer 22 and the lowest size fragment (116 bp) by SSR 16 & 18. Total of 71 alleles generated by using 24 SSR markers and the obtained PIC values. These findings indicate a high level of polymorphism among 6 chickpea mutants with one control genotype. Generally, genotypes have lower PIC value described that lower mutants and higher PIC values indicates higher mutants with higher genetic diversity.