Biocatalytic Synthesis of Corticosteroid Derivatives by Toad-Derived Steroid C21-Hydroxylase
CsCYP21A, a steroid 21-hydroxylase from <i>Bufo bufo gargarizans</i>, exhibits unprecedented sequential oxidations. Optimizing <i>Pichia pastoris</i> biotransformation conditions enhanced C21-hydroxylation selectivity, converting 14 substrates to 21-hydroxylated products, with 10 conversions of >80% and 4 yields of >80%. Hydrocortisone production reached 1.5 g L<sup>-1</sup> day<sup>-1</sup> with 100 g/L wet biomass. CsCYP21A's versatility enables integration into the synthesis of over 10 steroidal drugs, offering a sustainable biocatalytic platform for green pharmaceutical manufacturing.
Authors
- Xueqing Zhong
- Jia-Xu Qin
- Qunfei Zhao (ORCID: https://orcid.org/0000-0003-3115-7337)
- Qingli He (ORCID: https://orcid.org/0000-0001-9417-8958)
- Mengmeng Duan (ORCID: https://orcid.org/0000-0002-0753-6663)
- Guo‐Qiang Lin (ORCID: https://orcid.org/0000-0002-8209-5263)
Institutions
- Shanghai University of Traditional Chinese Medicine (CN)
Publication Details
- Journal
- Organic Letters
- Published
- 2025-04-17
- DOI
- https://doi.org/10.1021/acs.orglett.5c00639
- Citations
- 1
- Primary Topic
- Steroid Chemistry and Biochemistry
- Type
- article
- Field-Weighted Citation Impact
- 0.81
Funders
- Shanghai Science and Technology Development Foundation
- Shanghai Municipal Health Commission
- National Natural Science Foundation of China
- Shanghai University of Traditional Chinese Medicine