Greenhouse gas emissions reduction utilizing methane-oxidizing bacteria in critical growth stages of paddy

Paddy cultivation contributes to greenhouse gas (GHG) emissions in the agricultural sector.The consistency of the biological option to reduce GHG emissions is utilizing methane-oxidizing bacteria in paddy cultivation.This study aimed to obtain methane (CH4) and nitrous oxide (N2O) reduction by applying methane-oxidizing bacteria in soil incubation and the critical growth stages of paddy in paddy fields.The research was conducted at the greenhouse gas laboratory for soil incubation with four isolates (Amorphomonas oryzae (SI5), Ciceribacter sp.(OF4), Rhodanobacter rhizosphaerae (TH6), Bordetella petrii (BD4)), and combination bacterial consortium at the paddy field scale.The treatment consisted of applying bacterial consortium and organic matter at the field scale.The first factor: R0 = without bacterial application; R1 = SI5, OF4, BD4; R2 = SI5, TH6, OF4; R3 = SI5, BD4, Priestia aryabhattai.The second factor was organic matter addition; O0 = without manure and O1 = 2 t ha -1 farmyard manure.Applying four isolates had a positive effect in reducing N2O and CH4 concentrations in soil incubation.The SI5 reduced CH4 concentration by 22%, and BD4 showed a 22% N2O reduction during soil incubation.The bacterial consortium R3 was the best treatment to reduce total CH4 and N2O emissions at three critical growth stages of paddy and produced the highest total population of methanotrophs and denitrifying bacteria.Bacterial consortia are suggested as a potential strategy for promoting sustainable agriculture and environment.