Protocol for the isolation and characterization of murine hematopoietic stem and progenitor cell-derived extracellular vesicles
Hematopoietic stem cells (HSCs) maintain their self-renewal capacity in an autocrine manner through hematopoietic stem and progenitor cell (HSPC)-derived extracellular vesicles (EVs). Here, we present a protocol for the isolation and characterization of EVs from HSPCs starting from an in vivo murine model. We describe steps for murine bone marrow isolation, HSPC staining and sorting, HSPC-derived EV isolation, size and concentration characterization, and EV visualization and marker description. For complete details on the use and execution of this protocol, please refer to Bonora et al.<sup>1</sup>.
Authors
- Ilaria Zanolla (ORCID: https://orcid.org/0000-0002-7395-787X)
- Keisuke Ito (ORCID: https://orcid.org/0000-0002-6702-9735)
- Barbara Zavan (ORCID: https://orcid.org/0000-0002-4779-4456)
- Claudia Morganti (ORCID: https://orcid.org/0000-0003-0527-4505)
- Federica Zanotti (ORCID: https://orcid.org/0000-0002-8721-8162)
- Paolo Pinton (ORCID: https://orcid.org/0000-0001-7108-6508)
- Massimo Bonora (ORCID: https://orcid.org/0000-0002-5752-3318)
- Letizia Ferroni (ORCID: https://orcid.org/0000-0002-5457-0338)
Institutions
- Albert Einstein College of Medicine (US)
- University of Ferrara (IT)
- Maria Cecilia Hospital (IT)
Publication Details
- Journal
- STAR Protocols
- Published
- 2025-04-17
- DOI
- https://doi.org/10.1016/j.xpro.2025.103778
- Citations
- 1
- Primary Topic
- Extracellular vesicles in disease
- Type
- article
- Field-Weighted Citation Impact
- 0.81