Rotating mirror short-wave infrared hyperspectral imaging system: Characterization and applications

Hyperspectral imaging (HSI) is a powerful analytical tool with broad applicability across diverse fields, including agriculture, biomedicine, and industry. This article introduces a new custom hyperspectral device that operates in the SWIR (Short-Wave InfraRed) range (900-1700 nm). Based on a previously tested design featuring a rotating mirror, this device opens new possibilities for the physicochemical characterization of tissues and materials. This equipment provides up to 5 nm spectral resolution and 176 . 8 × 111 . 36 μ m 2 spatial resolution, without moving the equipment or the sample. Key features of the device are detailed, and a dual validation method is presented: using reference spectra to their concentrations and using reference concentrations to estimate their spectra. Validation is carried out through the quantitative analysis of liquid mixtures and the qualitative analysis of biological specimens. The proposed HSI system extends the capabilities of the technique into the SWIR range, offering strong potential for its application in diverse biomedical and industrial scenarios. • A compact hyperspectral system in the SWIR range (900-1700 nm) with a rotating mirror is presented, providing high spectral and spatial resolution. • Organic-sample method shows uniform spectral response in SWIR characterization. • A new reflectance model is proposed that accounts for optical path corrections and surface reflections. • Case studies highlight system’s power in biological sample imaging.