Expression in HEK293 cells, purification, and characterization of recombinant human α-glucosidases
In mammals, intestinal α-glucosidase exists within maltase-glucoamylase (MGAM) and sucrase-isomaltase (SI) complexes. Here, we purified recombinant human MGAM and SI from human embryonic kidney 293 cells using Flag tag-based affinity chromatography. Steady-state kinetic analysis at pH 6.0 and 37 °C showed that the maltase activities of MGAM and SI were higher than their sucrase and isomaltase activities.
Authors
- Noriyuki Kioka (ORCID: https://orcid.org/0000-0002-2708-537X)
- Kazumichi M. Nishida
- Kiyoshi Yasukawa (ORCID: https://orcid.org/0000-0001-7748-6043)
- Masaaki Ito (ORCID: https://orcid.org/0000-0002-1101-2707)
- Teisuke Takita
- Toshiyuki Kimura (ORCID: https://orcid.org/0000-0001-5928-371X)
- Yasuhisa Kimura (ORCID: https://orcid.org/0000-0001-6466-1324)
- So Nishimoto
Institutions
- National Agriculture and Food Research Organization (JP)
- Kyoto University (JP)
- National Institute of Technology Okinawa College (JP)
- Okinawa Prefecture (JP)
Publication Details
- Journal
- Bioscience Biotechnology and Biochemistry
- Published
- 2025-04-16
- DOI
- https://doi.org/10.1093/bbb/zbaf055
- Citations
- 2
- Primary Topic
- Digestive system and related health
- Type
- article
- Field-Weighted Citation Impact
- 3.63