Microtubule-driven cell shape changes and actomyosin flow synergize to position the centrosome

The regulation of centrosome position is critical to the alignment of intracellular structures with extracellular cues. The exact nature and spatial distribution of the mechanical forces that balance at the centrosome are unknown. Here, we used laser-based nanoablations in adherent cells and found that forces along microtubules were damped by their anchoring to the actin network, rendering them ineffective in moving the microtubule aster. In contrast, the actomyosin contractile network was responsible for the generation of a centripetal flow that robustly drives the centrosome toward the geometrical center of the cell, even in the absence of microtubules. Unexpectedly, we discovered that the remodeling of cell shape around the centrosome was instrumental in aster centering. The radial array of microtubules and cytoplasmic dyneins appeared to direct this reorganization. This revised view of the respective roles of actin and microtubules in centrosome positioning offers a new perspective for understanding the establishment of cell polarity.