Comparison between two Techniques to evaluate the effect of fluconazole on some fungi isolated from laboratory instruments

The purpose of this investigation was to review the antifungal activity of fluconazole on some fungi isolated from laboratory instruments. The study mediated twenty samples taken from laboratories in Al-Karkh University for Science, College of Science, department of microbiology in Baghdad. The sensitivity of fluconazole was tested using disk diffusion procedure and food poisoning technique for the following molds (Penicillium, Ulocladium, Fusarium, Aspergillus niger, Aspergillus fumigates, Botrytis, and Rhizopus). The inhibitory effect of fluconazole using the food poisoning technique was the highest against Fusarium and Botrytis in contrast with Rhizopus, which showed resistance to fluconazole. The sensitivity of the isolated mold was tested using the disk diffusion procedure for the antifungal drugs (fluconazole 10-μg disks). All the tested molds showed resistance to the fluconazole disk. Fluconazole and other azole medications block the enzyme 14-a-demethvlase, which transforms lanosterol into ergosterol, which is the main building block of fungal cell membranes.