Investigation of vaginal colonization of bacterial infections in women

Background and Objectives: Vaginal microbiota is responsible for up to 70% reproductive tract infections in women.Bacterial vaginosis is a significant vaginal infection involved in replacing the normal flora of lactobacilli with aerobic and anaerobic opportunistic bacteria.This study was conducted to determine the prevalence of bacterial vaginosis in women of reproductive age with the complaint of vaginal discharge in Pakistan.METHODOLOGY: A total of 100 sterile vaginal swab samples were collected from women aging between 15-50 years at Allied Hospital Faisalabad.The swabs were inoculated on sheep blood and MacConkey agar for bacterial isolation.Gram staining and biochemical testing were done to identify the bacterial species.All resulted isolates were subjected to antibacterial sensitivity testing by Kirby Bauer diffusion method.RESULTS: High bacterial colonization rate (75%) was recorded in women among age group 26-35 years.Bacteria isolated in this study were Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pyogenes, Streptococcus pneumonia, Escherichia coli, Micrococcus spp.Acinetobacter bauminii, Peptostreptococcus spp.and Enterobacter faecalis.The bacterial isolates showed highest resistance against tetracycline, ofloxacin and ciprofloxacin and were sensitive to meropenem, gentamycin, clindamycin and kanamycin.CONCLUSION: : In conclusion our findings evidenced that bacterial vaginosis is prevalent in women and showed resistant patterns to clinical antibiotics.