Evaluation of the bactericidal activity of a laboratory sample of Argocide

A laboratory sample of the drug Argocide has been developed, which includes the drug Argovit (LLC NPTS Vector-Vita, Novosibirsk, Russia) containing silver nanoparticles and an enzyme preparation for the preservation of feed Biosib Acid (NPO Sibbiopharm, Berdsk, Russia). Strains of microorganisms and their isolates were used for the study – St. aureus ATCC 25953, St. epidermidis ATCC 14990, Str. agalactiae ATCC12386, Str. disgalactiae ATCC27957, Str. pyogenes ATCC 19615, S. enteritidis ATCC 13076, Pr. vulgaris ATCC 6380, P. aeruginosa ATCC 27853, E. Coli ATCC 25922. The sensitivity of microorganisms to antibacterial substances and their combinations was determined by the method of sequential serial dilutions in BCH. The studied drugs were an aqueous 10.0% dilution of Argovit, a 10.0% aqueous solution of Biosib Acid and a 10% laboratory sample of Argocide, consisting of 10.0% Argovit and 10.0% Biosib Acid in equal amounts. An assessment of the bactericidal activity of Argovit, Biosib Acid and a laboratory sample of Argocide allowed us to build a series of decreasing antimicrobial activity, to establish where the highest indicators were noted in Str. disgalactiae ATCC27957 (0.01 mcg/ml) St. aureus ATCC 25953, Str. agalactiae ATCC 12386, E. coli ATCC 25922 (0.05 mcg/ml) St epidermidis ATCC 14990 (0.1 mcg/ml) S. enteritidis ATCC 13076 (0.2 mcg/ml) Kl. pneumoniae 71, Pr. vulgaris ATCC 6380 (0.5 mcg/ml). The bactericidal activity of the studied drugs was significantly lower (by 2-20 times) for field isolates of the reference strains Klebsiella pneumoniae (2.0 mcg/ml) S. enteritidis (1.25 mcg/ml) Pr. vulgaris (1.0 mcg/ml) St. aureus (1.0 mcg/ml) Str. disgalactiae (0.1 mcg/ml) St. epidermidis (0.1 mcg/ml) Str. agalactiae (0.05 mcg/ml) E. coli (0.05 mcg/ml), which can be explained by prolonged contact with antibacterial substances in the animal’s body.