Nonanol, an Induced Biomolecule Produced by <i>Bacillus atrophaeus</i> NMB01 During Interaction With <i>Phytophthora infestans</i> Can be Explored as a Novel Formulation for the Management of Late Blight of Potatoes

Phytophthora infestans, the pathogen responsible for late blight, continues to pose a significant risk to worldwide potato cultivation, including its historical impact during the Irish Potato Famine. Traditional management relies heavily on synthetic fungicides, but their prolonged use has led to fungicide resistance and environmental concerns. This study examines the potential of the bacterial endophyte Bacillus atrophaeus NMB01 as a biocontrol agent against P. infestans. Six biomolecules produced by B. atrophaeus NMB01 were docked against 15 P. infestans protein targets, with 1-nonanol, glafenine hydrochloride, and mucic acid showing high binding affinity. Wet lab assays confirmed that 1-nonanol inhibited P. infestans mycelial growth by 78% at 2 ppm. Molecular dynamics simulations validated the stability of these interactions. A tri-trophic interaction study identified additional volatile and non-volatile organic compounds (VOCs/NVOCs), with minocycline and doxazosin exhibiting strong binding across all targets. Transcriptome analysis of P. infestans exposed to 1-nonanol revealed differential gene expression, with upregulated genes linked to stress responses and downregulated genes, such as TAR1, cysteine synthase, and glutathione transferase, presenting novel antifungal targets. This study highlights 1-nonanol as a promising eco-friendly alternative to conventional fungicides, offering a sustainable solution for managing late blight and advancing potato cultivation resilience.