Imaged Capillary Isoelectric Focusing (icIEF) Platform for Characterization of Charge Variants of Adeno-Associated Virus (AAV) Capsids and Impact on their Transduction Efficiency
These PTMs have biological consequences reflected in the diminished expression of the protein of interest in vitro. This cIEF method successfully analyzed a variety of AAV serotypes, and increasing trends of acidic variants led to reduced in vitro potency.
Authors
- Soumita Das (ORCID: https://orcid.org/0000-0003-3895-3643)
- Jonathan Hill (ORCID: https://orcid.org/0000-0003-2801-0449)
- Dhimiter Bello (ORCID: https://orcid.org/0000-0003-2402-5651)
- Jonghan Kim
- B. Hoyle (ORCID: https://orcid.org/0009-0009-3013-2673)
Institutions
- University of Massachusetts Lowell (US)
- Solidus Biosciences (United States) (US)
Publication Details
- Journal
- Current Gene Therapy
- Published
- 2025-04-16
- DOI
- https://doi.org/10.2174/0115665232369708250327081305
- Citations
- 2
- Primary Topic
- Viral gastroenteritis research and epidemiology
- Type
- article
- Field-Weighted Citation Impact
- 2.79