Glycerophospholipids Analysis Using Conventional <sup>1</sup>H–<sup>31</sup>P HMBC and Its Application in Revealing the Characteristics of 18 Seaweeds
Glycerophospholipids (GPLs) are typical membrane lipids with important physiological and pharmacological functions, but little is known about their distribution characteristics in economic seaweeds. This study established a systematic evaluation method, including conventional 2D <sup>1</sup>H-<sup>31</sup>P heteronuclear multiple bond correlation (HMBC) NMR for identifying GPLs and 1D <sup>31</sup>P NMR for determining the contents, which were then applied to reveal subclasses characteristics of GPLs in 18 economic seaweeds in Asia. The results indicated that phosphatidylglycerol (PG) was the characteristic GPL subclass distributed in all of the 18 seaweeds (ranging from 0.08 to 1.75‰), generally with higher contents than that of known terrestrial plants. The characteristic chemical shifts of protons in the GPLs headgroup (H-1') together with protons in the backbone (H-3) were summarized through <sup>1</sup>H-<sup>31</sup>P HMBC spectrum, which were helpful in assigning GPL subclasses.
Authors
- Hongbing Liu (ORCID: https://orcid.org/0000-0001-5530-1490)
- Aiping Chen (ORCID: https://orcid.org/0000-0003-2639-2797)
- Shanpeng Ma (ORCID: https://orcid.org/0000-0003-2451-6557)
- Xiuli Zhang (ORCID: https://orcid.org/0000-0002-4898-6599)
- Cong Wang (ORCID: https://orcid.org/0000-0002-2269-4766)
- Depeng Wang (ORCID: https://orcid.org/0000-0003-0939-1004)
Institutions
- Qingdao National Laboratory for Marine Science and Technology (CN)
- Quality Research (US)
- ZheJiang Institute For Food and Drug Control (CN)
- Ocean University of China (CN)
Publication Details
- Journal
- Journal of Agricultural and Food Chemistry
- Published
- 2025-04-16
- DOI
- https://doi.org/10.1021/acs.jafc.5c01794
- Primary Topic
- Metabolomics and Mass Spectrometry Studies
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Key Research and Development Program of China