The Effect of Adipocyte-Derived Stem Cell Media on Stem Cell Markers in Neuroblastoma Cells

Objective: Different effects of stem cell media have been reported in different types of cancer.Neuroblastoma (NB) is the most common extracranial tumour of childhood.To be knowledgeable about pathophysiology of the disease is crucial for its effective treatment.The study aimed to determine the efficacy of human adipocytederived stem cell media (ADSC-M) on stem cell markers in NB cells.Method: In the study, SH-SY5Y/N-MYC(-) and KELLY/N-MYC(+) cells were grown and adipocyte-derived stem cell conditioned medium was applied.Flow cytometry was used to identify changes in the expressions of stem cell markers in cells' own proliferative, and normal conditioned medium (NCM) and ADSC-M.Results: While CD14, CD33, CD44, CD73 expressions disappeared in KELLY cells, CD34 levels in SH-SY5Y neuroblastoma cells decreased in ADSC-M rather than NCM.CD90 and CD106 expressions increased in KELLY and decreased in SH-SHY5Y when incubated in ADSC-M.Expression levels of HLA-ABC decreased twofold in SH-SY5Y when incubated in ADSC-M rather than NCM.Again, incubation of SH-SY5Y cells in ADSC-M decreased the expression of HLA-DR and CD45 down to undetectable levels compared to NCM. Conclusion:The study found that ADSC-M negatively impacted mesenchymal and adipocyte-derived stem cell markers, especially in N-MYC-positive KELLY neuroblastoma cells, indicating poor prognosis.