A LAMP Detection System Based on a Microfluidic Chip for Pyricularia grisea

As one of the major rice fungal diseases, blast poses a serious threat to the yield and quality of rice globally. It is caused by the pathogen <i>Pyricularia grisea</i>. Therefore, the development of rapid, accurate, and portable microfluidic detection system for <i>Pyricularia grisea</i> is important for the control of rice blast. This study presents an integrated microfluidic detection system for the rapid and sensitive detection of <i>Pyricularia grisea</i> using the LAMP detection method. The microfluidic detection system includes a microfluidic chip, a temperature control module, and an OpenMv camera. The micro-mixing channels with shear structures improve the mixing efficiency to about 98%. Flow-blocking valves are used to reduce reagent loss in the reaction chamber. The temperature control module is used to heat the reaction chamber, maintaining a stable temperature of 65 °C. The microfluidic chip detection chamber is used for image inspection using an OpenMv camera. The developed system can detect <i>Pyricularia grisea</i> in the range of 10 copies/μL-10⁵ copies/μL within 45 min. Specificity and interference experiments were performed on <i>Pyricularia grisea</i>, validating the method's good reliability. This LAMP detection system based on a microfluidic chip has strong potential in the early and effective detection of rice blast.