Effective polyclonal antibodies against the virulence-associated protein D (VapD) of Helicobacter pylori, obtained from recombinant VapD
Helicobacter pylori is a microorganism associated with serious gastric pathologies. This bacterium presents specific genes that encode for different virulence factors associated with the development of gastric disease. The VapD protein has rarely been studied, although it has been previously demonstrated its participation in the protection of Helicobacter pylori within gastric cells. In the present work, we document the protocols developed to generate the VapD recombinant protein and the subsequent production of polyclonal antibodies. Our research group faced several problems throughout the trials; however, all of them were successfully solved.
Authors
- Gabriela Delgado
- Alejandro Flores-Alanis (ORCID: https://orcid.org/0000-0001-5837-6076)
- Carlos Santiago-Olivares (ORCID: https://orcid.org/0000-0002-7078-7318)
- Víctor M. Luna-Pineda (ORCID: https://orcid.org/0000-0002-3078-9423)
- Nayeli Torres‐Ramírez (ORCID: https://orcid.org/0000-0002-7515-9740)
- María L. Escobar-Sánchez
- Rosario Morales-Espinosa (ORCID: https://orcid.org/0000-0003-2110-4397)
- Armando Cruz‐Rangel (ORCID: https://orcid.org/0000-0002-0113-0312)
- Denilson Guerrero-Mejía
Institutions
- Universidad Autónoma de Guerrero (MX)
- Hospital Infantil de México Federico Gómez (MX)
- National Institute of Genomic Medicine (MX)
- Universidad Nacional Autónoma de México (MX)
Publication Details
- Journal
- PLoS ONE
- Published
- 2025-04-16
- DOI
- https://doi.org/10.1371/journal.pone.0321455
- Citations
- 2
- Primary Topic
- Helicobacter pylori-related gastroenterology studies
- Type
- article
- Field-Weighted Citation Impact
- 4.01
Funders
- Consejo Nacional de Ciencia y Tecnología
- Dirección General de Asuntos del Personal Académico, Universidad Nacional Autónoma de México