A novel CRISPR–Cas12a-based fluorescence anisotropy method with a high signal-to-background ratio for sensitive biosensing
Here, a CRISPR-Cas12a system with high trans-cleavage ability integrating a DNA nanochain formed by DNA tetrahedrons with a large molecular mass was employed to enhance the signal-to-background ratio of the fluorescence anisotropy method for achieving sensitive detection of hepatitis B virus DNA.
Authors
- Yu Qin (ORCID: https://orcid.org/0000-0003-1130-7115)
- Kai Mao
- Shu Jun Zhen (ORCID: https://orcid.org/0000-0003-1236-5501)
- Cheng Zhi Huang (ORCID: https://orcid.org/0000-0002-1260-5934)
- Jia Xie
- Hongyan Zou
- Yuan Fang Li
Institutions
- Southwest University (CN)
Publication Details
- Journal
- Chemical Communications
- Published
- 2025-01-01
- DOI
- https://doi.org/10.1039/d5cc01565k
- Citations
- 2
- Primary Topic
- CRISPR and Genetic Engineering
- Type
- article
- Field-Weighted Citation Impact
- 1.61
Funders
- National Natural Science Foundation of China