Optimizing Cost-Effective gene expression phenotyping approaches in cattle using 3′ mRNA sequencing
This work demonstrates that 3' mRNA-Seq with Takara SMART-Seq v4 3' DE can provide an incredibly cost-effective (< 25 USD/sample) approach to quantifying molecular phenotypes (gene expression) while discovering sufficient variation for use in genotype imputation. Ongoing work is evaluating the accuracy of imputation and the ability of much larger datasets to predict individual animal phenotypes.
Authors
- A.G. Ríus (ORCID: https://orcid.org/0000-0001-5602-4990)
- Sonia J. Moisá (ORCID: https://orcid.org/0000-0003-1776-8721)
- Ruwaa I. Mohamed (ORCID: https://orcid.org/0000-0002-8950-5069)
- Jonathan E. Beever (ORCID: https://orcid.org/0000-0001-6156-0632)
- Taylor B. Ault-Seay (ORCID: https://orcid.org/0000-0002-3462-540X)
- Troy N. Rowan (ORCID: https://orcid.org/0000-0001-5347-7695)
Institutions
- University of Tennessee at Knoxville (US)
Publication Details
- Journal
- BMC Genomics
- Published
- 2025-04-16
- DOI
- https://doi.org/10.1186/s12864-025-11571-4
- Citations
- 2
- Primary Topic
- Genomics and Phylogenetic Studies
- Type
- article
- Field-Weighted Citation Impact
- 1.61
Funders
- U.S. Department of Agriculture
- Foundation for Food and Agriculture Research
- National Institute of Food and Agriculture