Protocol for in vivo assessment of glucose control and insulin secretion and sensitivity in the pig
The pig is a valuable animal model in diabetes research; however, standardized protocols are essential for evaluating in vivo metabolism. Here, we present a protocol for in vivo assessment of glucose control and insulin secretion and sensitivity in the pig. We describe steps for catheter implantation, testing of intravenous glucose tolerance, performance of hyperinsulinemic-euglycemic clamps (HECs) and hyperglycemic clamps (HGCs), and blood processing. We then detail procedures for analysis of plasma glucose, insulin, glucagon, and C-peptide concentrations as well as data analysis. For complete details on the use and execution of this protocol, please refer to Renner et al.<sup>1</sup> and Renner et al.<sup>2</sup>.
Authors
- Tove Lorenzen
- S.J. Koopmans (ORCID: https://orcid.org/0000-0001-8238-4770)
- Martin Hrabě de Angelis (ORCID: https://orcid.org/0000-0002-7898-2353)
- Birgit Rathkolb (ORCID: https://orcid.org/0000-0003-1239-0547)
- Benedikt Mathias Kessler (ORCID: https://orcid.org/0000-0002-8160-2446)
- Eckhard Wolf (ORCID: https://orcid.org/0000-0002-0430-9510)
- Andreas Blutke (ORCID: https://orcid.org/0000-0001-7824-2681)
- Simone Renner (ORCID: https://orcid.org/0000-0002-5866-4577)
- Anne von Thaden
- Berit Østergaard Christoffersen
- Armin Manfred Scholz (ORCID: https://orcid.org/0000-0001-7075-0067)
- Arne Hinrichs (ORCID: https://orcid.org/0000-0003-3022-5983)
- István Novák
- Yasmin Eckstein
Institutions
- Novo Nordisk (Denmark) (DK)
- German Center for Neurodegenerative Diseases (DE)
- Helmholtz Zentrum München (DE)
- LMU Klinikum (DE)
- Institute of Bioinformatics and Systems Biology (DE)
- Centre of Experimental Medicine of the Slovak Academy of Sciences (SK)
- Deutsches Diabetes-Zentrum e.V. (DE)
- German Center for Diabetes Research (DE)
- Heinrich Heine University Düsseldorf (DE)
- Ludwig-Maximilians-Universität München (DE)
- Wageningen University & Research (NL)
Publication Details
- Journal
- STAR Protocols
- Published
- 2025-04-16
- DOI
- https://doi.org/10.1016/j.xpro.2025.103774
- Citations
- 2
- Primary Topic
- Adipose Tissue and Metabolism
- Type
- article
- Field-Weighted Citation Impact
- 2.33
Funders
- Deutsche Forschungsgemeinschaft