High‐Speed Centrifugation Fails to Mitigate Severe Lipemic Interference in D‐dimer Measurement

Contrary to CLSI recommendations, high-speed centrifugation (HC) significantly reduced D-dimer concentrations in the lower plasma layer of severely turbid specimens (e.g., type II hyperlipidemia), failing to mitigate turbidity interference. This discrepancy may lead to misdiagnosis of high-risk thrombotic conditions, such as pancreatitis. The upper lipid layer, predominantly composed of chylomicrons, exhibits a strong binding affinity with D-dimer, further complicating accurate measurement. Direct dilution, however, may resolve measurement failures in severe LT samples by maintaining analyte integrity while eliminating lipid interference.