Impact of clinical preparation steps and use of sex-specific reference for accurate antibiotic monitoring in body fluids
Resonance Raman spectroscopy effectively quantifies piperacillin concentrations in urine. Freezing, centrifugation, and filtration during sample preparation influence drug concentration. Using sex-specific urine pools as references yields more accurate quantification results. These findings underscore the importance of considering sample processing effects and reference selection in TDM studies, offering insights for optimizing antibiotic dosing in critically ill patients. Further validation on a larger scale is warranted to confirm these observations.
Authors
- Jürgen Popp (ORCID: https://orcid.org/0000-0003-4257-593X)
- Torsten Frosch (ORCID: https://orcid.org/0000-0003-3358-8878)
- Stefan Hagel (ORCID: https://orcid.org/0000-0003-2999-6131)
- Mathias Wilhelm Pletz (ORCID: https://orcid.org/0000-0001-8157-2753)
- Christian Domes
- Timea Frosch (ORCID: https://orcid.org/0000-0001-8346-0253)
- Lisa Graul
Institutions
- Leibniz Institute of Photonic Technology (DE)
- Technische Universität Darmstadt (DE)
- Jena University Hospital (DE)
Publication Details
- Journal
- Communications Medicine
- Published
- 2025-04-16
- DOI
- https://doi.org/10.1038/s43856-025-00823-9
- Primary Topic
- Antibiotics Pharmacokinetics and Efficacy
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Bundesministerium für Bildung und Forschung