In Vitro Anti-Inflammatory Terpenoid Glycosides from the Seeds of Dolichos lablab

To further explore the anti-inflammatory components of the seeds of <i>Dolichos lablab</i> L., a comprehensive phytochemical investigation was conducted using diverse chromatographic and spectrometric technologies, as well as chemical reactions. As a result, ten previously unreported terpenoid glycosides, namely dolilabterpenosides A, B, C₁-C₃, D, E, and F₁-F₃ (<b>1</b>-<b>10</b>), along with four known analogues (<b>11</b>-<b>14</b>), initially identified from <i>Dolichos</i> genus, were obtained. In addition, the lipopolysaccharide (LPS)-induced RAW264.7 cell model was employed to detect the expression levels of nitric oxide (NO), inflammatory cytokines tumour necrosis factor (TNF)-α and interleukin (IL)-1β to assess the anti-inflammatory activities of the obtained compounds. The results of bioactive assay showed that compounds <b>1</b>, <b>4</b>-<b>7</b>, and <b>10</b>-<b>12</b> showed significant inhibitory activity on NO release in RAW264.7 cells in a dose-dependent manner, and all of them were demonstrated to inhibit the increase in TNF-α and IL-Iβ levels in the supernatant of RAW264.7 cells stimulated by LPS.