Immune Response to Bioluminescence Imaging Reporters in Murine Tumor Models
These findings demonstrate that the stable expression of click beetle green luciferase in cancer cells, in contrast to red-shifted firefly luciferase, has minimal immunogenicity and does not alter tumor development in immunocompetent mice. We report detailed characterization studies of bioluminescence reporter cells, providing essential considerations for their use in investigating tumor-immune interactions in syngeneic murine tumor models.
Authors
- Tracy Liu (ORCID: https://orcid.org/0000-0003-0671-8390)
- Angisha Basnet (ORCID: https://orcid.org/0000-0002-9000-9062)
- F. Heath Damron (ORCID: https://orcid.org/0000-0002-3140-402X)
- Dylan D. Thomas (ORCID: https://orcid.org/0000-0002-3610-1888)
- Kaitlyn M. Landreth
Institutions
- West Virginia University (US)
- West Virginia University Hospitals (US)
Publication Details
- Journal
- Molecular Imaging and Biology
- Published
- 2025-04-15
- DOI
- https://doi.org/10.1007/s11307-025-02010-7
- Primary Topic
- bioluminescence and chemiluminescence research
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- U.S. Department of Defense
- Lustgarten Foundation
- West Virginia University
- National Institutes of Health
- Cancer Institute, West Virginia University
- National Institute of General Medical Sciences
- Division of Graduate Education