Single cell RNAseq to identify subpopulations of glial progenitors in iPSC-derived oligodendroglial lineage cultures
Cellular heterogeneity is a common issue in differentiation protocols of oligodendrocytes (OLs) from human induced pluripotent stem cells. Our previous work described a novel method to generate OLs and highlighted the presence of glial progenitors. Here, we unravel the glial heterogeneity and characterize the response of isolated subpopulations to differentiation. This study provides a novel tool for studying the dynamics of glial development in vitro and on a transcriptomic level.
Authors
- Geneviève Bernard (ORCID: https://orcid.org/0000-0002-9634-2966)
- Thomas M. Durcan (ORCID: https://orcid.org/0000-0003-3942-1956)
- Valerio E. C. Piscopo (ORCID: https://orcid.org/0000-0002-0602-2839)
- Julien Sirois (ORCID: https://orcid.org/0000-0001-6606-4961)
- Jack P. Antel (ORCID: https://orcid.org/0000-0002-5148-0636)
- Taylor Goldsmith (ORCID: https://orcid.org/0009-0002-4247-9884)
- Alexandra Chapleau (ORCID: https://orcid.org/0000-0002-4741-1355)
- Gabriela J. Blaszczyk (ORCID: https://orcid.org/0000-0003-3329-9275)
Institutions
- Montreal Neurological Institute and Hospital (CA)
- McGill University Health Centre (CA)
- McGill Genome Centre (CA)
- McGill University (CA)
Publication Details
- Journal
- npj Systems Biology and Applications
- Published
- 2025-04-15
- DOI
- https://doi.org/10.1038/s41540-025-00515-z
- Citations
- 1
- Primary Topic
- Neuroinflammation and Neurodegeneration Mechanisms
- Type
- article
- Field-Weighted Citation Impact
- 0.84
Funders
- McGill University
- Canada First Research Excellence Fund
- Canadian Institutes of Health Research
- Fonds de Recherche du Québec - Santé