D‐amino acid oxidase as a chemogenetic tool for spatiotemporally controlled hydrogen peroxide production: The oxygen connection
High DAO activity can induce hypoxia because of increased oxygen consumption. Caution should be taken to prevent hypoxic conditions in cell culture experiments when using DAO. A provided spreadsheet is intended to help estimate oxygen levels in combination with OCR measurements.
Authors
- Laura L Bauer (ORCID: https://orcid.org/0000-0002-1435-8121)
- Dörthe M. Katschinski (ORCID: https://orcid.org/0000-0003-4630-9081)
- Diane de Zélicourt (ORCID: https://orcid.org/0000-0001-5300-1355)
- Anke Zieseniß (ORCID: https://orcid.org/0000-0003-2585-8444)
- Peter Rehling (ORCID: https://orcid.org/0000-0001-5661-5272)
- Wiebke Maurer (ORCID: https://orcid.org/0000-0003-3023-4345)
- Vartan Kurtcuoglu (ORCID: https://orcid.org/0000-0003-2665-0995)
- Maithily S. Nanadikar (ORCID: https://orcid.org/0000-0002-5557-8059)
- Yamin Chen (ORCID: https://orcid.org/0000-0002-1610-0650)
- Annette Hillemann
- V. G. Shanmuga Priya
Institutions
- University of Zurich (CH)
- Fraunhofer Institute for Translational Medicine and Pharmacology (DE)
- Universitätsmedizin Göttingen (DE)
- Max Planck Institute for Multidisciplinary Sciences (DE)
- University of Göttingen (DE)
Publication Details
- Journal
- British Journal of Pharmacology
- Published
- 2025-04-15
- DOI
- https://doi.org/10.1111/bph.70046
- Primary Topic
- Amino Acid Enzymes and Metabolism
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Science Foundation
- Deutsche Forschungsgemeinschaft
- Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung
- Bundesministerium für Bildung und Forschung
- China Scholarship Council