Antimicrobial Efficacy of Different Diabetic Toothpastes on Anaerobic Periodontal Pathogens: An In vitro Experimental Study

Abstract Background: Uncontrolled diabetes mellitus is a chronic metabolic disorder affecting multiple organs and organ systems. Poor oral hygiene results in periodontitis as well as impaired blood sugar control. Exploring the antimicrobial effect of different diabetic toothpaste on common periodontal pathogens helps to choose the ideal toothpaste for diabetics. Materials and Methods: Out of 3 toothpaste, randomly 2 diabetic toothpastes, Apollo Pharmacy Diabetic Sugar-Free Toothpaste and Colgate toothpaste for the oral health of diabetics compared with 1 Colgate toothpaste are tested against Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans , commonly implicated in periodontal diseases. Blood agar culture media and agar diffusion method were used for measuring the zone of inhibition, and minimum inhibitory concentration (MIC) was measured using McFarland turbidity for testing the antimicrobial activity of two anaerobic microbes. Results: Among all the 3 toothpastes, Apollo Pharmacy Diabetic Sugar-Free Toothpaste shows the highest zone of inhibition against P. gingivalis (21.0 ± 1.41) with P = 0.008. Minimum inhibitory concentration (MIC ) of apollo pharmacy diabetic sugar free toothpaste was 6.25 μL/mL for P. Gingivalisand it is less for A. Actinomycetecomitansthat is 0.8 μL/m. MIC for the Colgate toothpaste for the oral health of diabetics was 6.25 μL/mL for the both the bacteria. Conclusion: Apollo Pharmacy Diabetic Sugar-Free Toothpaste is most effective against P. gingivalis and A. actinomycetemcomitans .