Rutin Protects Pancreatic Islets Against Methylglyoxal-Induced Oxidative Stress and Elevates Insulin Secretion

Objective: Hyperglycemia is the main characteristic of diabetes, which leads to complications, including oxidative stress (OS).Pancreatic -cells are susceptible to damage against OS, which results in a disruption in insulin secretion.The current study focused on the antioxidant features of rutin (Rtn) flavonoid to prevent methylglyoxal (MG-)induced oxidative damage in pancreatic islets isolated from mice. Methods:After isolating islets from twenty-four male mice, we conducted two experimental parts, (1) with exposure to MG-and (2) without exposure to MG.Experiments were carried out in both culture media with 5.6-and 16.7-mM glucose concentrations.Islets were transferred to the culture medium and exposed to different substances.In the end, insulin secretion, antioxidant enzyme activities, and malondialdehyde (MDA) concentration were investigated by ELISA. Results:The reduced levels of insulin in MG-exposed islets (p=0.01,p<0.001 in 5.6-and 16.7-mM glucose concentration, respectively) were reversed by Rtn treatment.MG-increased MDA levels in 5.6-and 16.7-mM glucose concentrations (p<0.001 for 5.6 mM and p=0.005 for 16.7 mM.Also, we observed a remarkable decrease in the activities of catalase, superoxide dismutase, and glutathione peroxidase due to 300 M MG-exposure in islets, in 5.6-and 16.7-mM glucose concentrations.Rtn at doses of 1 and 2 M significantly reduced MDA levels.Moreover, Rtn had beneficial effects on antioxidant activities.Ama: Hiperglisemi, diyabetin temel zelliidir ve oksidatif stres (OS) dahil olmak zere eitli komplikasyonlara yol aar.Pankreatik -hcreleri OS'ye kar hassastr ve bu durum inslin salglanmasnda bozulmaya neden olur.Bu alma, rutin (Rtn) flavonoidinin antioksidan zelliklerine odaklanarak, metilglioksal (MG) kaynakl oksidatif hasara kar farelerden izole edilen pankreatik adacklar koruma potansiyelini incelemektedir.Yntemler: Yirmi drt erkek fareden adacklar izole edildikten sonra, iki deneysel blm gerekletirildi: (1) MG'ye maruz braklan grup ve 2. MG'ye maruz braklmayan grup.Deneyler, 5.6 mM ve 16.7 mM glukoz konsantrasyonlarna sahip kltr ortamlarnda yapld.Adacklar kltr ortamna aktarld ve farkl maddelere maruz brakld.Son olarak, inslin salglanmas, antioksidan enzim aktiviteleri ve malondialdehit (MDA) konsantrasyonu ELISA yntemiyle incelendi.Bulgular: MG'ye maruz braklan adacklarda azalan inslin seviyeleri (5.6 mM glukoz konsantrasyonu iin p=0.01, 16.7 mM glukoz konsantrasyonu iin p<0.001),Rtn tedavisi ile tersine evrildi.MG, 5.6 mM ve 16.7 mM glukoz konsantrasyonlarnda MDA seviyelerini artrd (5.6 mM iin p<0.001 ve 16.7 mM iin p=0.005).Ayrca,