Deciphering cargo contents in extracellular vesicles of Candida haemulonii var. vulnera
The <i>Candida haemulonii</i> complex poses significant clinical challenges due to its intrinsic resistance to conventional antifungal therapies and diagnostic complexities. This research explores the cargo of EVs released by <i>C. haemulonii</i> var. <i>vulnera</i>, revealing a selective mechanism for exporting proteins and RNA molecules critical to the fungus' adaptation and survival in diverse environments. Proteomic and RNA analyses identified molecules involved in metabolic reprogramming, immune modulation, and stress response. These findings highlight the role of EVs in mediating host-pathogen interactions, facilitating immune evasion, and contributing to fungal virulence. Understanding the EV cargo expands our knowledge of fungal biology and underscores the therapeutic potential of targeting EV-associated molecules in antifungal strategies.
Authors
- Caroline Patini Rezende (ORCID: https://orcid.org/0000-0001-8263-6958)
- Renan Eugênio Araujo Piraine (ORCID: https://orcid.org/0000-0001-6650-8286)
- Arnaldo Lopes Colombo (ORCID: https://orcid.org/0000-0003-0793-8491)
- Fausto Bruno dos Reis Almeida (ORCID: https://orcid.org/0000-0002-3782-3698)
- Patrick Wellington da Silva dos Santos (ORCID: https://orcid.org/0000-0001-5722-023X)
- Tamires A. Bitencourt (ORCID: https://orcid.org/0000-0001-7373-3780)
- Vítor M. Faça (ORCID: https://orcid.org/0000-0003-3205-7944)
- Ana Paula Masson (ORCID: https://orcid.org/0000-0002-9081-8345)
- André Moreira Pessoni (ORCID: https://orcid.org/0000-0003-4549-1387)
- Lysangela R. Alves (ORCID: https://orcid.org/0000-0002-1972-2658)
- Vinícius Farias Campos (ORCID: https://orcid.org/0000-0003-2119-293X)
- B. Oliveira (ORCID: https://orcid.org/0000-0002-6433-9551)
- Antônio D. Pagano
Institutions
- Universidade de Ribeirão Preto (BR)
- Universidade Federal de Pelotas (BR)
- Universidade de São Paulo (BR)
- Fundação Carlos Chagas (BR)
- Clinics Hospital of Ribeirão Preto (BR)
- Universidade Federal de São Paulo (BR)
- Medical Research Council (GB)
Publication Details
- Journal
- Computational and Structural Biotechnology Journal
- Published
- 2025-01-01
- DOI
- https://doi.org/10.1016/j.csbj.2025.04.014
- Citations
- 1
- Primary Topic
- Antifungal resistance and susceptibility
- Type
- article
- Field-Weighted Citation Impact
- 1.40
Funders
- University of Exeter
- Fundação de Amparo à Pesquisa do Estado de São Paulo
- Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
- Conselho Nacional de Desenvolvimento Científico e Tecnológico
- Universidade de São Paulo
- Fundação de Amparo à Pesquisa do Estado da Bahia
- Fundação de Apoio ao Ensino, Pesquisa e Assistência do Hospital das Clínicas da Faculdade de Medicina de Ribeirão Preto da Universidade de São Paulo