A new 2D Zn(II)-based coordination polymer as highly sensitive selective fluorescent probe for of glutathione

Fluorescence-based sensing has emerged as an effective strategy for detecting biomolecules with high sensitivity and selectivity. In this study, a new Zn(II)-based coordination polymer (CP), {Zn(Cl-L)(DPP)]·1.5DMF} n (1) , was synthesized and explored as a fluorescence sensor for the detection of glutathione (GSH). The coordination polymer was synthesized via a solvothermal method , and its structural characterization was confirmed using X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FT-IR), and X-ray photoelectron spectroscopy (XPS). CP 1 exhibited strong quenched upon interaction with GSH, indicating a strong binding affinity . Mechanistic investigations, including Inner Filter Effect (IFE) correction and XPS analysis, confirmed that the fluorescence quenching was primarily attributed to IFE and possible electron transfer interactions between 1 and GSH. The quenching constant ( K sv ) was determined to be 0.993 × 10 −3 M −1 , and the detection limit (LOD) was calculated as 2.38 × 10 −5 M, highlighting the high sensitivity of the sensor. Additionally, fluorescence lifetime analysis showed a decrease from 41.2 μs to 33.1 μs upon GSH interaction, indicating significant changes in the excited-state molecular environment. The practical applicability of this sensing method was evaluated in real water samples, including deionized (DI) water, tap water, and river water , with excellent recovery rates ranging from 93.04 % to 100.98 % and relative standard deviations ( RSD ) below 2.2 %, confirming the reliability and accuracy of the method. This study establishes 1 as an effective, selective, and sensitive fluorescence probe for GSH detection, with potential applications in environmental monitoring, biomedical diagnostics, and clinical research . CP 1 shows an effective, selective, and sensitive fluorescence probe for GSH detection, with potential applications in environmental monitoring, biomedical diagnostics, and clinical research. • The new Zn(II)-CP 1 used luminescent sensor for high detection of glutathione (GSH). • The turn-off mechanism explained and discussed in details. • The practical applicability of this sensing approach was also explored.