Tailored 3D microphantoms: An essential tool for quantitative phase tomography analysis of organoids
We present a novel approach for benchmarking and validating quantitative phase tomography (QPT) systems using three-dimensional microphantoms. These microphantoms, crafted from biological and imaging data, replicate the optical and structural properties of multicellular biological samples. Their fabrication featuring refractive index modulation at sub-micrometer details is enabled by two-photon polymerization. We showcase the effectiveness of our technique via a round-robin test of healthy and tumoral liver organoid phantoms across three different QPT systems. This test reveals sample- and system-dependent errors in measuring dry mass and morphology. This approach constitutes a development of super phantoms for QPT — test objects that exist in both digital and physical form, replicate both the morphology and relevant aspects of physiology in specimens under healthy or diseased conditions, and underpin the assessment and refinement of imaging technologies and methodologies prior to clinical application.
Authors
- Guillaume Godefroy (ORCID: https://orcid.org/0000-0002-0538-7967)
- Sylvia Desissaire (ORCID: https://orcid.org/0000-0002-7227-8031)
- Lionel Hervé (ORCID: https://orcid.org/0000-0002-2944-5498)
- Kiran Padmanabhan (ORCID: https://orcid.org/0000-0001-7020-1682)
- Małgorzata Kujawińska (ORCID: https://orcid.org/0000-0001-6521-6951)
- Wojciech Krauze (ORCID: https://orcid.org/0000-0002-5248-3986)
- Michał Ziemczonok (ORCID: https://orcid.org/0000-0002-4802-1105)
- Arkadiusz Kuś (ORCID: https://orcid.org/0000-0001-8009-4841)
- Damien Sery
- Cécile Fiche
- Marie Fackeure
- Chiara Paviolo (ORCID: https://orcid.org/0009-0006-8763-8470)
- J. M. Neri
Institutions
- Université Claude Bernard Lyon 1 (FR)
- Warsaw University of Technology (PL)
- Centre National de la Recherche Scientifique (FR)
- Commissariat à l'Énergie Atomique et aux Énergies Alternatives (FR)
- CEA Grenoble (FR)
- Direction de la Recherche Technologique (FR)
- Institut de Génomique Fonctionnelle de Lyon (FR)
- Laboratoire d'Électronique des Technologies de l'Information (FR)
- Université Grenoble Alpes (FR)
Publication Details
- Journal
- Journal of Applied Biomedicine
- Published
- 2025-04-01
- DOI
- https://doi.org/10.1016/j.bbe.2025.03.003
- Citations
- 2
- Primary Topic
- Digital Holography and Microscopy
- Type
- article
- Field-Weighted Citation Impact
- 2.49