Chiral sensing combined with nuclease activity assay to track Cas9 dynamics in solution: ROA and CPL study
Chiroptical studies of the SpyCas9 protein are extremely rare. Nondestructive methods are needed to characterize its active ribonucleoprotein form. Using Raman optical activity (ROA) and circularly polarized luminescence (CPL), we present a new approach to detect key biomolecules involved in CRISPR-Cas technology while preserving their original nucleolytic activity.
Authors
- Magdalena Klimek‐Chodacka (ORCID: https://orcid.org/0000-0002-6254-9280)
- Tomasz Oleszkiewicz (ORCID: https://orcid.org/0000-0001-9115-3499)
- Grzegorz Zając (ORCID: https://orcid.org/0000-0003-4090-9334)
- Josef Kapitán (ORCID: https://orcid.org/0000-0002-1916-9186)
- Agnieszka Domagała (ORCID: https://orcid.org/0000-0003-2940-4933)
- Monika Halat (ORCID: https://orcid.org/0000-0002-9119-7341)
- Rafał Barański (ORCID: https://orcid.org/0000-0001-9276-3870)
Institutions
- Jagiellonian University (PL)
- University of Agriculture in Krakow (PL)
- Palacký University Olomouc (CZ)
Publication Details
- Journal
- Chemical Communications
- Published
- 2025-01-01
- DOI
- https://doi.org/10.1039/d5cc00971e
- Citations
- 1
- Primary Topic
- Advanced biosensing and bioanalysis techniques
- Type
- article
- Field-Weighted Citation Impact
- 0.81
Funders
- Narodowe Centrum Nauki