Detection of Virulence Genes in Klebsiella pneu-moniae and Staphylococcus aureus Isolated from Urinary Catheterised Patients

Background: One of the most predominant health-related illnesses universally is catheter-associated urinary tract infection (CAUTI). CAU-TIs constitute over 50% of all hospital-acquired infections. Majority of the healthcare-acquired urinary tract infections result from cathetertubes implantation.Objective: To reveal the existence and frequency of the fimH, Ycfm,clfA, and Cap5 genes in two species isolated from patients with urinarycatheters.Methods: During the period from November 2023 to February 2024, a total of 50 samples of urine were collected from patients with uri-nary catheters at Tikrit Teaching Hospital and private clinics in Salah Al-Deen province, Iraq. Different antibiotics were used to examine theantibiotics sensitivity test for bacterial isolates.Results: Among the participants, 30 (60%) were female and 20 (40%)were male, with ages ranging between 18 to 80 years and a mean age of 42.06± 14.6 years. Sensitivity for isolates of S. aureus to antibiot-ics was 100% for amoxicillin, 66.6% for trimethoprim, and 44.4% for gentamicin and nitrofurantoin. 11.1% of isolates showed resistanceto ciprofloxacin. Among the K. pneumoniae isolates, 66.6 % exhibited resistance to amoxicillin and trimethoprim, while 11.1% showed resis-tance to ciprofloxacin. Molecular analysis was done for 2 isolates of S. aureus and 2 isolates of K. pneumoniae based on their high resistanceto antibiotics. The results revealed that the clfA gene was found in100% of S.aureus isolates, while the Cap5 gene was found in 50% ofS.aureus isolates. Also, the result unveiled that 100% of K. pneumoniaeisolates produced FimH-1 and Ycfm genes.Conclusion: S. aureus and K. pneumoniae are common causes of urinarytract infections in patients with urinary catheters. Virulence genes havean important role in the pathogenicity of S. aureus and K.pneumoniaein patients with urinary catheters. How to cite this article:Khuder H S, Khudhur S S. Detection ofVirulence Genes in Klebsiella pneumoniaeand Staphylococcus aureus Isolated fromUrinary Catheterised Patients. J Commun Dis.2025;57(1):222-230. DOI: https://doi.org/10.24321/0019.5138.202529