Spatial proteomics in translational and clinical research

Spatial proteomics offers unprecedented insights into the localization, quantity, and interactions of proteins within cells and tissues, thereby enabling researchers and clinicians to map protein networks with high precision. This powerful approach is currently revolutionizing our understanding of cellular organization and function (Method of the Year 2024: spatial proteomics, 2024), and providing deeper insights into how protein distribution and dynamics contribute to physiological and pathological processes. Here, we discuss recent advances in targeted and untargeted (exploratory) spatial proteomics, shed light on emerging multiscale approaches for tissue profiling, and highlight their translational potential.