Protective effects of hydrogen (H<sub>2</sub>) on boar sperm upon freeze-thaw process
BACKGROUND: Antioxidant is important to prevent ROS attack in boar sperm during the freezing-thawing process. OBJECTIVE: To study whether hydrogen (H 2 ) could improve the survival of boar sperm upon the freeze-thaw process. MATERIALS AND METHODS: The effects of hydrogen in pre-diluent, freezing extender and thawing solution were investigated. Sperm viability, progressive motility, acrosomal integrity, mitochondrial activity, as well as the levels of malonaldehyde (MDA) and total antioxidant capacity (T-AOC) were measured. RESULTS: Hydrogen in pre-diluent, freezing extender and thawing solution significantly improved sperm quality parameters (including sperm viability, progressive motility, acrosomal integrity and mitochondrial activity), increased total antioxidant capacity (T-AOC) and decreased malondialdehyde (MDA) of post-thawed boar sperm. The use of hydrogen in all three solutions improved the quality of post-thawed boar sperm. CONCLUSION: Hydrogen protects boar sperm against ROS-induced damages during cooling and the freezing-thawing process.
Authors
- Shushan Zhang (ORCID: https://orcid.org/0000-0002-6762-1931)
- Jianjun Dai (ORCID: https://orcid.org/0000-0002-8126-7430)
- Yunhai Zhang
- Defu Zhang (ORCID: https://orcid.org/0000-0002-4041-2363)
- Shang Ren
- Caifeng Wu
Institutions
- Anhui Agricultural University (CN)
- Shanghai Academy of Agricultural Sciences (CN)
Publication Details
- Journal
- Cryoletters
- Published
- 2025-04-14
- DOI
- https://doi.org/10.54680/fr25310110512
- Primary Topic
- Hydrogen's biological and therapeutic effects
- Type
- article
- Field-Weighted Citation Impact
- 0.00