Development of In Vitro Potency Methods to Replace In Vivo Tests for Enterovirus 71 Inactivated Vaccine (Human Diploid Cell-Based/Vero Cell-Based)
Based on conformational epitope-targeting neutralizing MAbs, manufacturer-specific IVRP methods, which were sensitive to process variations and correlated with in vivo results, have been established. IVRP methods provide a reliable, animal-free alternative for EV71 vaccine batch release.
Authors
- Longfa Xu (ORCID: https://orcid.org/0000-0001-6307-5665)
- Qingbing Zheng (ORCID: https://orcid.org/0000-0002-7516-9965)
- Zhengling Liu
- Qunying Mao (ORCID: https://orcid.org/0000-0002-8967-4436)
- Yadong Li (ORCID: https://orcid.org/0000-0002-7166-3261)
- Wenhui Wang (ORCID: https://orcid.org/0000-0003-3258-1885)
- Yi Li (ORCID: https://orcid.org/0000-0002-2092-4505)
- Jun Li (ORCID: https://orcid.org/0009-0002-9250-6705)
- Jing Guo (ORCID: https://orcid.org/0000-0001-8982-1275)
- Desheng Wu (ORCID: https://orcid.org/0000-0001-6201-9047)
- Fan Gao (ORCID: https://orcid.org/0000-0002-7949-0828)
- Qian He (ORCID: https://orcid.org/0000-0003-4346-9899)
- Kaiwen Wang (ORCID: https://orcid.org/0000-0003-3857-4790)
- Xiao Ma (ORCID: https://orcid.org/0000-0003-0044-531X)
- Zhenglun Liang (ORCID: https://orcid.org/0000-0002-6740-0864)
- Huiyi Li (ORCID: https://orcid.org/0000-0001-9574-7251)
- Jing Li (ORCID: https://orcid.org/0000-0003-3684-3491)
- Zejun Wang (ORCID: https://orcid.org/0000-0001-5076-3215)
- Dan Yu (ORCID: https://orcid.org/0000-0003-2114-1200)
- Rui Zhu (ORCID: https://orcid.org/0000-0002-6543-7380)
- Yong Zhao (ORCID: https://orcid.org/0000-0003-1001-0512)
- X.B. Li
- Xuanxuan Zhang
- Tong Cheng (ORCID: https://orcid.org/0000-0002-1638-6214)
Institutions
- Xiamen University (CN)
- Chinese Academy of Medical Sciences & Peking Union Medical College (CN)
- Sinovac Biotech (CN)
- Wuhan Institute of Bioengineering (CN)
- National Institutes for Food and Drug Control (CN)
Publication Details
- Journal
- Vaccines
- Published
- 2025-04-13
- DOI
- https://doi.org/10.3390/vaccines13040404
- Citations
- 1
- Primary Topic
- Viral Infections and Immunology Research
- Type
- article
- Field-Weighted Citation Impact
- 1.39
Funders
- National Natural Science Foundation of China
- Natural Science Foundation of Beijing Municipality