Phytochemical Analysis and Biological activity raw materials of Oxytropis rosea Bunge (Fabaceae) from the Flora of Kyrgyzstan

Oxytropis rosea Bunge is a herbaceous plant from the family Fabaceae, growing in Central Asia and Kazakhstan, Western Tien Shan and mountainous areas of Kyrgyzstan. Macroscopic analysis of grass and roots of O. rosea, qualitative analysis using chemical reactions and TLC, dynamics of flavonoids, tannins and ascorbic acid accumulation using instrumental and chemical methods of analysis in raw materials harvested in different phases of vegetation were carried out. The predominance of the content of the studied groups in the terrestrial part of O. rosea was revealed. The predominance of the amount of tannins was revealed in the following order: vegetation period - flowering period - fruiting period. An increase in the content of flavonoids in grass and roots during flowering and then a decrease during fruiting was found. High content of ascorbic acid in grass and roots during vegetation period with subsequent decrease by fruiting period was revealed. Antioxidant activity of grass and roots of O. rosea was determined using two in vitro screening techniques. Aqueous extracts from the grass of O. rosea with the highest content of flavonoids, ascorbic acid and tannins had the maximum antioxidant activity. It was found that the level of antioxidant activity correlates with the concentration of flavonoids in the studied raw materials of O. rosea. The obtained data indicate the possibility of using the grass of O. rosea harvested during flowering as a source of phenolic substances with antioxidant activity. The experimental research was carried out as part of the implementation of the federal program of strategic academic leadership "Priority 2030".