ANALYSIS OF THE CONTENT OF HEAVY METAL SALTS IN MILK AND THEIR INTERACTION WITH ENZYMES

The article substantiates the relevance of the application aimed at studying the content of heavy metals in milk and their interaction with enzymes. It should be noted that research is currently underway to study the interaction of enzymes with heavy metals in environmental objects and in a living organism. It is known that certain heavy metals both inhibit the activity of individual enzymes and stimulate their activity. For example, studies have been conducted proving that cadmium stimulates peroxidase activity, but inhibits catalase activity regardless of the concentration of this element in the soil. The main purpose of the study was to study the interaction of heavy metal salts with redox groups of the milk enzyme. When studying the interaction of salts of lead, cadmium, arsenic and mercury with catalase and peroxidase, it was found that the salts of the studied heavy metals have only an inhibitory effect on the enzyme catalase and peroxidase. At the same time, salts of elements such as mercury and cadmium have the greatest inhibitory effect, followed by lead and arsenic. The restoration of milk peroxidase activity was established after the introduction of oatmeal and additional filtration by qualitative reaction. With a lead content of 0.06 mg/l in the milk sample, catalase activity was 4.98 E, and after filtration, its activity increased to 8.3 E. With a cadmium content of 0.20 mg/l in milk, catalase activity before filtration was 3.32 E, after filtration it increased to 5.81 E. With a mercury content of 0.011 mg/l in milk, catalase activity before filtration was 2.49 E, after filtration it increased to 4.15 E. With an arsenic content of 0.11 mg/l in milk, catalase activity was 6.64 E, after filtration it increased to 8.3 E. Currently, research is underway to study the interaction of enzymes with heavy metals in environmental objects, in a living organism, but the issue of research on the interaction of heavy metals with milk enzymes has not been studied.