Simultaneous Determination of Mangiferin and Neomangiferin by Electrochemical Detection based on CuBTC and GO-COOH Modified Glassy Carbon Electrode

An electrochemical sensor for the simultaneous detection of mangiferin (MAN) and neomangiferin (NEO) was developed using CuBTC and GO-COOH through an electroreduction method for the first time. The electrochemical properties of the CuBTC@ErGO-COOH/GCE and the electrochemical behavior of MAN and NEO on this sensor were investigated. Several factors were investigated, including the concentration of CuBTC, pH value, accumulation time and scanning rate using cyclic voltammetry. The electrochemical reaction processes of MAN and NEO on the CuBTC@ErGO-COOH/GCE were inferred. An electroanalytical method was developed for the simultaneous detection of MAN and NEO using linear sweep voltammetry, allowing for precise and sensitive quantification of both compounds. The electrode reaction processes of MAN and NEO on the CuBTC@ErGO-COOH/GCE were both determined to be diffusion-controlled. The linear ranges for MAN and NEO were established from 0.1 ∼ 8 μM, with the detection limits of 8.1 nM and 17 nM (S/N = 3), respectively. The sensor demonstrated good repeatability, reproducibility, stability and anti-interference ability. Furthermore, it was successfully applied to the simultaneous detection of MAN and NEO in urine and serum samples with satisfactory recovery.