Improved methyl supply for 5-methyltetrahydrofolate production in E. coli through a novel C1 transfer pathway
We successfully engineered E. coli by introducing a novel exogenous C1 metabolic pathway to augment the methyl donor pool essential for the biosynthesis of 5-MTHF. Further metabolic optimizations, including the enhancement of the THF precursor flux and the elimination of competing degradation pathways, developed a recombinant strain with significantly increased yield, which paves the way for industrial production of 5-MTHF.
Authors
- Rubing Zhang (ORCID: https://orcid.org/0000-0003-0985-7900)
- Wen Liu (ORCID: https://orcid.org/0000-0002-8376-7842)
- Tao Cheng (ORCID: https://orcid.org/0000-0002-4184-0730)
- Yiting Li (ORCID: https://orcid.org/0000-0002-2484-246X)
- Jing Guo (ORCID: https://orcid.org/0000-0003-2106-3169)
- Wei Lü (ORCID: https://orcid.org/0000-0003-4043-6047)
- Mo Xian (ORCID: https://orcid.org/0000-0001-6300-0786)
Institutions
- Qingdao Institute of Bioenergy and Bioprocess Technology (CN)
- University of Chinese Academy of Sciences (CN)
Publication Details
- Journal
- Microbial Cell Factories
- Published
- 2025-04-15
- DOI
- https://doi.org/10.1186/s12934-025-02707-y
- Citations
- 3
- Primary Topic
- Folate and B Vitamins Research
- Type
- article
- Field-Weighted Citation Impact
- 5.00
Funders
- Taishan Scholar Foundation of Shandong Province
- China National Tobacco Corporation
- Key Technology Research and Development Program of Shandong