Optimization of shoot multiplication in satoimo <i>(Colocasia esculenta</i> L.) using 6-benzylaminopurine (BAP) and thidiazuron (TDZ) in vitro

Abstract Satoimo is an introduced plant that has the potential as a food source. One alternative method of propagating satoimo seeds is using tissue culture technology. The purpose of this study was to determine how combining BAP and Thidiazuron impacts the development of satoimo shoots in vitro. The research design used was a completely randomized factorial with two factors. The initial factor was BAP (0, 0.5, 2.5, 5 ppm) and the subsequent nd factor was TDZ (0, 1.5 ppm). The study found that the use of growth regulators BAP and Thidiazuron can help increase the number of shoots. The addition of Thidiazuron produced a higher number of shoots compared to BAP, although the height of the shoots was not maximal. The combination of BAP 2.5 ppm with Thidiazuron 1.5 ppm produced the highest number of shoots, and subculture in media without growth regulators increased the height of the shoots. The addition of Thidiazuron must be balanced with other types of cytokinins to be able to increase maximum shoot multiplication.