Post-cleavage target residence determines asymmetry in non-homologous end joining of Cas12a-induced DNA double strand breaks
The asymmetric retention of CRISPR-LbCas12a at DSB ends suppresses c-NHEJ at PPEs, not at PDEs. This unique repair mechanism can be utilized in the CIPDEL strategy, offering a potentially better alternative for homology-directed targeted integration.
Authors
- Rugang Tian (ORCID: https://orcid.org/0000-0002-1648-7226)
- Anyong Xie (ORCID: https://orcid.org/0000-0002-6608-2550)
- Kun-Ming Liu
- Ruo-Dan Chen
- Shiming Xu (ORCID: https://orcid.org/0000-0003-4999-3499)
- Ruirui Jiang (ORCID: https://orcid.org/0000-0002-7863-6186)
- Ji‐Fan Hu
- Yue Wang (ORCID: https://orcid.org/0000-0002-1211-2822)
- Yulong Wang (ORCID: https://orcid.org/0009-0005-4080-5019)
- Peiheng Han (ORCID: https://orcid.org/0000-0003-2772-2380)
- Chunyi Yang (ORCID: https://orcid.org/0009-0001-9906-3479)
- Sicheng Liu (ORCID: https://orcid.org/0000-0002-9147-9082)
- Yi Yang (ORCID: https://orcid.org/0000-0002-0045-215X)
- Yi-Li Feng (ORCID: https://orcid.org/0000-0002-7143-9956)
Institutions
- Sir Run Run Shaw Hospital (CN)
- Zhejiang Cancer Hospital (CN)
- Inner Mongolia Academy of Agricultural & Animal Husbandry Sciences (CN)
- Zhejiang Hospital (CN)
- Hangzhou Xixi hospital (CN)
- Zhejiang University of Technology (CN)
- Zhejiang University (CN)
Publication Details
- Journal
- Genome biology
- Published
- 2025-04-14
- DOI
- https://doi.org/10.1186/s13059-025-03567-w
- Citations
- 1
- Primary Topic
- CRISPR and Genetic Engineering
- Type
- article
- Field-Weighted Citation Impact
- 0.81
Funders
- National Natural Science Foundation of China
- Zhejiang University
- National Science and Technology Major Project
- National Major Science and Technology Projects of China