Defining the networks that connect RNase III and RNase J-mediated regulation of primary and specialized metabolism in <i>Streptomyces venezuelae</i>

RNA processing and metabolism are mediated by ribonucleases and are fundamental processes in all cells. In the morphologically complex and metabolically sophisticated <i>Streptomyces</i> bacteria, RNase III and RNase J influence both development and metabolism through poorly understood mechanisms. Here, we show that both ribonucleases are required for the proper expression of the BldD developmental pathway and contribute to the control of chloramphenicol production, with an interesting connection to phosphate regulation for RNase III. Additionally, we show that both RNases have the potential to impact translation through distinct mechanisms and can function cooperatively in degrading specific transcripts. This study advances our understanding of RNases in <i>Streptomyces</i> biology by providing insight into distinct contributions made by these enzymes and the intriguing interplay between them.