Comparative Evaluation of the Effect of Grape Seed Extract, Strawberry Yogurt, Dente91 (nHAP, Lactoferrin and Xylitol), and Ozone on Enamel Remineralization of Posterior Teeth: An In Vitro Study

Background: Dental caries causes loss of mineral content and requires remineralization. To counteract the negative effects of fluoride, nonfluoride-based remineralizing products have evolved. Objective: To evaluate and compare the effect of grape seed extract, strawberry yogurt, Dente91^® (nHAP, lactoferrin, and xylitol), and ozone oil on enamel remineralization of posterior teeth. Materials and methods: Sixty sound extracted posterior permanent teeth that were selected were immersed in a demineralizing solution. The samples were subsequently categorized into four groups, each subjected to a different chemical treatment over a period of 21 days: group I with grape seed extract (GSE), group II with strawberry yogurt, group III with Dente91^®, and group IV with ozone. The effects were assessed using a digital analytical scale, Vickers microhardness tester, and scanning electron microscopy-energy dispersive X-ray analyzer (SEM-EDX). Statistical analysis was performed using paired <i>t</i>-test, one-way analysis of variance (ANOVA), and Tukey's honest significant difference (HSD) <i>post hoc</i> test. Results: All four groups delivered significant remineralization, with the highest mean weight difference and surface microhardness in group IV (ozone). SEM images and EDX revealed maximum mineral content in the ozone group, followed by strawberry yogurt, grape seed extract (GSE), and Dente91^®. Conclusion: All four agents—GSE, strawberry yogurt, Dente91^®, and ozone—showed significant remineralization; however, ozone proved to show the maximum results.