Transcriptional Profiling to Assess the Effects of Biological Stimulant Atlanticell Micomix on Tomato Seedlings Under Salt Stress

Recent environmental changes in the Mediterranean region, attributable to anthropogenic climate change, present a substantial challenge to the adaptive evaluation of crops and the development of novel improvement strategies. In this study, we established a hydroponic tomato cultivation protocol under in vitro conditions to analyze the transcriptomic profile of seedlings exposed to salinity stress. The study also examined the impact of Atlanticell Micomix, a biological stimulant derived from a mixture of mycorrhizal microorganisms and rhizobacteria, on plant growth and development under standard conditions and in response to moderate salinity. Our transcriptomic analysis indicated a differential effect of biostimulant inoculation compared to the effect induced by salinity stress, involving genes such as <i>GOX3</i> or <i>DIR1</i>, which are associated with the plant's defense response to adverse conditions. In addition, the presence of a cross-regulatory module between jasmonic acid and auxin, involving potential orthologs of <i>IAA29</i> and <i>JAZ</i>, was proposed. The application of the biostimulant demonstrated a potential priming effect on the tomato seedlings, which might be useful in reversing the transcriptomic effects caused by salt stress. A comprehensive analysis of the pathways differentially affected by the treatments facilitates further investigation into the mechanisms underlying these effects.