Studying The Effects of Microvascular Transplantation on Tissue in A Limb Ischemia Model

Vascular occlusion-induced ischemia can occur in any part of the body, causing oxygen deprivation and microvascular dysfunction, which restricts blood flow to muscles, tissues, and organs.The severity of ischemia depends on whether the vessel is partially or completely occluded, with symptoms varying based on the degree of blood flow restriction.Objectives: To study the effects of microvascular grafting surgery on tissue in a limb ischemia model. Methods:Forty male Wistar rats (Rattus norvegicus, body weight: 200-300g, age: 8weeks) were obtained from the Biomedical Institute and Experimental Animal Center of the National Institute of Medical Sciences.The rats were randomly divided into two groups: Control Group (n=10): Underwent direct end-to-end vascular grafting of a 0.3-0.5mmdiameter artery in the right femur.Experimental Group (n=30): Underwent end-to-end microvascular grafting using donor vessels preserved for 3 days and 7 days. Results:In the limb ischemia model, in the end-to-end anastomosis of allogeneic vascular transplantation, the tissue cell ischemia-reperfusion injury recovery ability of the vascular anastomosis preserved for 3 days was better than that of the vascular anastomosis preserved for 7 days. Conclusion:In the limb ischemia model, microvascular transplantation surgery showed different degrees of tissue edema, uneven cell distribution, tissue necrosis and other phenomena, which were more obvious in the 7-day ischemia group.When selecting vascular transplantation anastomosis, through our comparative study, it is best to choose blood vessels with a shelf life of less than 3 days.