Molecular screening of black currant cultivars and selected forms developed at the I.V. Michurin Federal Scientific Center for resistance to gall mite

Background. The gall mite ( Cecidophyopsis ribis Westw.) is a dangerous pest of black currant ( Ribes nigrum ), therefore the development of cultivars resistant to C. ribis is an important trend in many breeding programs. The objective of the study was to identify alleles of the gall mite resistance Ce gene in black currant cultivars and selected seedlings released at the I.V. Michurin Federal Scientific Center. Materials and methods. The tested material included 22 cultivars and 41 selected forms of black currant. The gall mite resistance allele of the Ce gene was identified using diagnostic DNA markers: GMresa and Ce-AFLP. Results and conclusion. The studied black currant cultivars were characterized by the absence of the GMresa and Ce-AFLP markers (recessive homozygous genotype cece ). Among the analyzed selected forms of black currant, the dominant resistance allele Ce was identified in 51.2% of forms. The results obtained for the GMresa and Ce -AFLP markers showed 100% matching in the distribution of target amplicons within the studied sample. Promising genetic sources of black currant resistance to C. ribis protected by the Ce gene were identified: 1448-6-49 (762-5-82 × ‘Ekzotika’), 1511-4-68 (977-31-54 × 747-1270), 34-320 (‘Shalunya’ × ‘Gratsiya’), 35-4-135 (‘Gratsiya’ × ‘Sensey’), 35-4-148 (‘Gratsiya’ × ‘Chernavka’), 35-5-4 (‘Kipiana’ × ‘Divo Zvyaginoy’), 35-5-36 (‘Kipiana’ × ‘Chernavka’), and 35-5-147 (1448-6-49 × ‘Divo Zvyaginoy’), with a heterozygous genotype ( Cece ), as well as seedlings 31-1-72 (1511-4-68 × ‘Lyuchiya’), 31-2-33, 31-2-35 (‘Kipiana’ × open pollination), 34-2-36 (‘Gratsiya’ × open pollination), 35-6-138 (1448-6-49 × self-pollination), 38-10-1, 38-10-5, 38-10-7, 38-10-9, 38-10-13, 38-10-16, and 38-10-17 (‘Gratsiya’ × ‘Dar Smolyaninovoy’), with a heterozygous or dominant homozygous genotype ( Cece or CeCe ).