Utilization of high-resolution mass spectrometry and data-independent analysis to track the monoclonal antibody spatial stability
This study reports the advantages of using combination of <sup>13</sup>C isotopically labeled MS-cleavable cross-linkers and data-independent mass spectrometry analysis for the automated identification of cross-linked products and thus monitoring the structural rearrangement of protein structure.
Authors
- Valerie Prochazkova
- Václav Matoušek (ORCID: https://orcid.org/0000-0002-6227-810X)
- Petr Novák (ORCID: https://orcid.org/0000-0001-8688-529X)
- Zdeněk Kukačka (ORCID: https://orcid.org/0000-0001-7569-843X)
- Michal Rosůlek (ORCID: https://orcid.org/0000-0002-2426-7928)
- Michael Karpisek
Institutions
- Czech Academy of Sciences (CZ)
- Charles University (CZ)
Publication Details
- Journal
- Expert Review of Proteomics
- Published
- 2025-04-13
- DOI
- https://doi.org/10.1080/14789450.2025.2492763
- Primary Topic
- Mass Spectrometry Techniques and Applications
- Type
- article
- Field-Weighted Citation Impact
- 0.00